Related Experiment Video
Updated: Feb 5, 2026
10:34
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Published on: April 14, 2010
16.0K
Dynamic Expression of Genes Involved in Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
P J E Uijtdewilligen1, E M Versteeg1, E M A van de Westerlo1
1Department of Biochemistry, Radboud University Medical Center, Radboud Institute for Molecular Life Sciences, Nijmegen, Netherlands.
Biomed Research International
|September 20, 2018
Summary
Glycosaminoglycans (GAGs) are vital for cell signaling and development. Skin development involves dynamic gene expression in GAG metabolism, influencing growth factor interactions and tissue homeostasis.
Area of Science:
- Biochemistry
- Developmental Biology
- Molecular Biology
Background:
- Glycosaminoglycans (GAGs) are crucial for cell signaling, embryonic development, and maintaining adult homeostasis.
- Proteoglycans and GAGs play significant roles in regulating growth factor activity.
Purpose of the Study:
- To analyze the expression patterns of genes involved in GAG metabolism during skin development.
- To investigate the relationship between GAG-associated gene expression and growth factor handling.
Main Methods:
- Microarray analysis and real-time quantitative PCR were employed to assess gene expression.
- GAG-related genes were categorized based on their function in GAG homeostasis.
Main Results:
- Highly dynamic up- and down-regulations were observed across all categories of GAG-related genes during skin development.
- Differential expression of GAG-modifying isoenzymes, core proteins, and growth factors was noted.
- Mice models with altered heparanase or C5 epimerase expression showed limited differential gene expression.
Conclusions:
- Skin development is characterized by complex and dynamic expression of GAG-associated genes.
- These changes likely reflect quantitative and qualitative alterations in GAGs/proteoglycans, impacting growth factor binding and signaling.
Related Concept Videos
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
01:42What is Gene Expression?
196.8K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.8K
01:36What is Gene Expression?
11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
01:05Proteoglycans
4.9K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
4.9K
02:51mRNA Stability and Gene Expression
6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.7K