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Updated: Feb 3, 2026

Longitudinal Intravital Imaging Through Clear Silicone Windows
Published on: January 5, 2022
Challenges and Opportunities for Intravital Near-Infrared Fluorescence Imaging Technology in the Second Transparency
Chunyan Li1,2, Qiangbin Wang1,2
1CAS Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine and i-Lab, CAS Center for Excellence in Brain Science , Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123 China.
Abstract:
The past decade has witnessed rapid technological development on nanoscale probes and imaging optics in the second near-infrared transparency window (NIR-II, 1000-1700 nm). These methods hold great promise for biomedical applications due to their deep penetration through tissues and high fidelity of images. However, applications of these techniques in biomedical research and translational medicine will require a number of issues to be addressed. In this Perspective, we examine the technical challenges for intravital NIR-II fluorescence imaging technology and discuss where the development of this cutting-edge technique fits in the future.
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