INFLUENCE OF H2S DONOR ON CHANGES IN THE CELL CYCLE AND THE APOPTOSIS PROCESS INDUCED BY DICLOFENAC IN THE RAT...

The purpose of this work was to study the effect of concomitant administration of sodium hydrosulfide and diclofenac sodium on the parameters cell cycle of the gastric mucosa cells by DNA flow cytometry. The experiments were performed on 24 white rats of Wistar straints. The parameters of the cell cycle of cells of the gastric mucosa of rats were determined by the method of flow-through DNA-cytometry. of mucosal cells of the stomach of rats was determined by DNA flow cytometry. It was established that NaHS monotherapy in the conditional therapeutic dose of 1.5 mg/kg ip (1/20 LD50) did not influence on DNA fragmentation and the parameters of the gastric mucosal cell cycle in the experimental rats. The sign of this was the lack of statistically significant differences in the parameters studied in comparison with the intact group. The data obtained suggested of the safety of H2S when used at the conditional therapeutic dose. The combined administration of NaHS and diclofenac sodium neutralized the negative effects of a non-steroidal anti-inflammatory agent on cell cycle indicators. An indication of the anti-apoptotic effect of NaHS was statistically significant reduction in number of cell events in the SUB-G0G1 interval by 1.68 times. The growth of BP, phase S and G2 + M indicators was also recorded. Therefore, the concomitant administration of diclofenac sodium and donor hydrogen sulfide reduced the number of cells with fragmented DNA and normalized regenerative properties of the mucous membrane of the stomach.