[HISTOLOGICAL AND IMMUNOHISTOCHEMICAL PROFILE OF THE MYOCARDIUM DURING ALCOHOL INTOXICATION IN THE EXPERIMENT]

The purpose of this study was to identify early structural and conjugated with them molecular changes in the myocardium, stroma and blood vessels under experimental alcohol intoxication, with histological and immunohistological methods. The experiments were carried out on white male rats with initial body weight 180-200gg. Accordingly, specific tasks were carried out 2 series of experiments: I series - acute alcohol intoxication, II series - chronic alcohol intoxication. The study of the both myocardium ventricles at different regimes of ethanol consumption revealed changes in cardiomyocytes and extracellular matrix - basal membrane, connective tissue and vessels. Microcirculation disorder, after 3 hours of ethanol intraperitoneal injection leads to acute myocardial hypoxia, and sarcoplasm vacuolization. Under chronic (30 days) ethanol intoxication, microcirculatory disturbances are aggravate, however, it becomes important the damage of the contractile apparatus in the form of a spectrum of changes: the myolysis, the vacuolization of the sarcoplasm. At the same time, in the wall of small arteries and between myofibrils structures identical to type IV collagen appear. The increase of Vimentin expression, in our experiment, indicates the starting of myocardial damage dynamic mechanisms, realized by the type of focal cardiosclerosis. Data on immunohistochemistry of p53 revealed that under our experimental conditions, the homeostasis of the cardiovascular tissue has changed. The initiation of cardiosclerosis can also be considered as the manifestation of p53 effects, as a factor forming a critical point of intramyocardial regulation disturbance.