VITAMINS D AND E COMBINED EFFECT ON THE RAT MYOCARDIUM UNDER ALLOXAN-INDUCED DIABETES

It is commonly known that in diabetes mellitus the risk of cardiovascular diseases and mortality increases. The purpose of this study was to determine the specific features of the morphological restructuring of the myocardium in the early stage of experimental diabetes and use the combined impact of powerful antioxidants (Vitamin C and Vitamin E) on the rat myocardium in conditions of alloxan-induced diabetes. The experimental animals were divided into three groups: 40 rats were the target, and 20 rats were the control. Target rats were divided into 2 groups: 1) group consisted of 20 rats with alloxan-induced diabetes; 2) group of 20 rats, which after confirmation of alloxan-induced diabetes, during 4 weeks were given intramuscularly vitamins C and E. The material was studied by histological and electron microscopic methods of investigation In the early stage of DM development, structural alterations in the cardiomyocytes and microcirculatory channels can be observed in the heart. Aggregation and agglutination of red blood cells and endothelial cell destruction were found in some vessels. In the study the result of combined therapy of Vitamin C and Vitamin E is a decrease in blood glucose levels, increases of endothelial proliferation, promoting the formation of new capillaries and further reduction of the structural changes of cardiomyocytes. Apperently through its antioxidative and anti-inflammatory activity Vitamins C and E stabilizes the metabolism processes in the vascular system and consequently, improvement of the organ function.