MORPHOLOGICAL CHARACTERISTICS OF ANGIOGENESIS IN ADENOCARCINOMA OF COLON

The study of the process of angiogenesis and microcirculation with the help of modern methods of morphological research including type and maturity of the tumor, location and is considered a problem of special importance and value. The purpose of this research is to study the particularity of angiogenesis and microcirculation in colon adenocarcinomas. The human tissue samples of colon adenocarcinoma (19 cases, 40 observation sites) were studied by light microscopic, electron- microscopic, electron-radiographic and immunohistochemical methods. For electron-radiographic studies - the sections of tissue were incubated separately in radioactive precursor 3H - thymidine and 3H - uridine. For immohistochemical studies markers CD34 and VEGF-A were used. According to analyzed data, de novo hyperplasia of endotheliocytes was detected by positive reaction on VEGF-A and CD34 markers in adenocarcinoma vascular structures. Special interest provide the VEGF-A and CD34 expression in stromal components. There is also an increase DNA synthesis in adenocarcinoma. Intra-tumor neoangiogenesis and changes in microcirculation are one of the most important factors regulating the processes of proliferation of atypical cells. The data obtained makes it possible to judge the prognosis, metastasis, and also the biopotential of neoplastic progression.