Antimicrobial agent isolated from Coptidis rhizome extract incubated with Rhodococcus sp. strain BD7100

Area of Science:

• Natural Product Chemistry
• Microbiology
• Pharmacology

Background:

• Coptidis rhizome (CR) is a traditional herbal medicine containing protoberberine alkaloids.
• Rhodococcus sp. strain BD7100 degrades berberine (BBR), a component found in CR.
• CR extract incubated with strain BD7100 exhibited antibacterial activity.

Purpose of the Study:

• To isolate and identify novel compounds from CR extract incubated with strain BD7100.
• To elucidate the structure of the isolated compound.
• To evaluate the antimicrobial activity of the isolated compound.

Main Methods:

• Incubation of CR extract with Rhodococcus sp. strain BD7100 in Luria-Bertani (LB) medium.
• Isolation of coptirhoquinone A (1) using chromatographic techniques.
• Structure elucidation using Nuclear Magnetic Resonance (NMR) and Mass Spectrometry (MS).
• Total synthesis of coptirhoquinone A.
• Antimicrobial activity testing against bacteria, fungi, and Pythium sp.

Main Results:

• Coptirhoquinone A (1) was successfully isolated from the incubated CR extract.
• The structure of coptirhoquinone A was determined through NMR and MS analysis.
• Total synthesis of coptirhoquinone A was achieved.
• Compound 1 demonstrated antimicrobial activity against a range of microorganisms.

Conclusions:

• Coptirhoquinone A is a novel compound isolated from CR incubated with strain BD7100.
• The study provides a new avenue for discovering antibacterial agents from herbal medicines.
• Coptirhoquinone A exhibits broad-spectrum antimicrobial properties.