Related Experiment Video
Updated: Feb 2, 2026
06:19
Author Spotlight: Exploring the Role of Ion Channels in Cancer: Characterization and Potential Treatment Approaches
Published on: June 16, 2023
3.9K
Aminoethane Sulfonic Acid Magnesium Salt Inhibits Ca2+ Entry Through NMDA Receptor Ion Channel In Vitro
E A Turovsky1, E V Blinova2, E V Semeleva3
1Institute of Cell Biophysics, Russian Academy of Science, Pushchino, Russia.
Bulletin of Experimental Biology and Medicine
|November 13, 2018
Summary
Magnesium bis-aminoethanesulfonate, a potential cerebroprotective agent, inhibits NMDA receptor activity and reduces intracellular calcium levels in rat neuroglial cells. These neuroprotective effects were found to be reversible.
Area of Science:
- Neuroscience
- Pharmacology
- Cell Biology
Background:
- Intracellular calcium concentration is critical for neuronal function and survival.
- NMDA receptors play a key role in excitotoxicity and neuronal damage.
- Cerebroprotective agents are sought to mitigate neurological injury.
Purpose of the Study:
- To investigate the effect of magnesium bis-aminoethanesulfonate (FS-LKhT-317) on intracellular calcium concentration in rat hippocampal neuroglial cells.
- To determine the impact of FS-LKhT-317 on NMDA receptor activity.
Main Methods:
- Fluorescent imaging technique on primary neuroglial cell cultures from Sprague-Dawley rat hippocampus.
- Application of NMDA in a magnesium-free medium to assess receptor activity.
- Evaluation of calcium (Ca2+) signal amplitude and pulses.
Main Results:
- Magnesium bis-aminoethanesulfonate demonstrated a pronounced inhibitory effect on intracellular calcium concentration at concentrations ≥50 μM.
- The agent suppressed NMDA receptor activity.
- Observed effects, including decreased Ca2+ signal amplitude and inhibited Ca2+ pulses, were reversible or partially reversible, particularly in the absence of magnesium.
Conclusions:
- Magnesium bis-aminoethanesulfonate exhibits cerebroprotective potential by inhibiting NMDA receptor-mediated calcium influx.
- The compound's ability to modulate intracellular calcium levels suggests a therapeutic role in conditions involving excitotoxicity.
- The reversible nature of the effects warrants further investigation into its pharmacological profile and clinical applications.
Related Concept Videos
01:19Ion Channels
91.4K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.4K
02:54Ions as Acids and Bases
26.4K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.4K
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
4.0K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.0K
02:02Responses to Salt Stress
14.6K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.6K
01:24Non-gated Ion Channels
8.2K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
8.2K
04:08Determining the pH of Salt Solutions
48.1K
The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution. In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
48.1K