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Updated: Feb 2, 2026

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
Published on: March 15, 2019
[Preparation and identification of anti-HaCHT4 mouse polyclonal antibody]
Abudunasier Maimaitiaili1, Xinrong Gu1, Ji Ma1
1College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China.
Abstract:
Objective The group IV chitinase from Helicoverpa armigera (HaCHT4)was expressed in prokaryotic and its polyclonal antibody against HaCHT4 was prepared. Methods The fusion protein His-HaCHT4 was expressed in E.coli and purified the target protein by Ni-column, and immunized the mice by footpad and subcutaneous injection. The titer of polyclonal antibody against His-HaCHT4 was determined by ELISA after four immunizations. The immunological specificity of the anti His-HaCHT4 was assayed by Western blot analysis. Results Fusion protein His-HaCHT4 was purified successfully and the titer of mouse anti His-HaCHT4 polyclonal antibody was higher than 1:204 800. The results of Western blot showed that the anti His-HaCHT4 polyclonal antibody was able to bind the fusion protein His-HaCHT4, and also specifically recognize the natural HaCHT4 in Helicoverpa armigera, but not the protein in Tenebrio molitor. Conclusion His-HaCHT4 was successfully expressed in prokaryotic and purified, and its mouse anti His-HaCHT4 polyclonal antibody was prepared.
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