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The extracellular matrix composition of the monkey optic nerve head
J C Morrison1, J A Jerdan, N L L'Hernault
1Glaucoma Service, Wilmer Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland.
Investigative Ophthalmology & Visual Science
|July 1, 1988
Summary
This study mapped key proteins in cynomolgus monkey optic nerve heads. Findings reveal their structural roles and potential impact on optic nerve health and glaucoma susceptibility.
Area of Science:
- Ophthalmology
- Neuroscience
- Biochemistry
Background:
- The optic nerve head's structural integrity is crucial for vision.
- Understanding the extracellular matrix composition is vital for optic nerve health.
Purpose of the Study:
- To immunohistochemically map laminin, heparan sulfate proteoglycan, and collagen types I, III, and IV in cynomolgus monkey optic nerve heads.
- To elucidate the structural roles of these matrix components and their implications for optic nerve function.
Main Methods:
- Immunohistochemistry using the avidin-biotin-peroxidase complex technique.
- Analysis of optic nerve head tissue from cynomolgus monkeys.
Main Results:
- Collagen types I and III are located in the scleral lamina cribrosa, septa, pia mater, and blood vessel walls, providing structural support.
- Collagen type IV, laminin, and heparan sulfate proteoglycans are found at basement membranes of vascular and glial cells, and along laminar plates and septa.
- These proteins are localized to basement membranes of vascular endothelial and glial cells within the optic nerve head.
Conclusions:
- The distribution of these matrix proteins suggests specific roles in structural support and axonal maintenance within the optic nerve head.
- Alterations in these components may affect optic nerve function and susceptibility to conditions like elevated intraocular pressure and glaucoma.