Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tree of Life - Bacteria, Archaea, Eukaryotes02:40

The Tree of Life - Bacteria, Archaea, Eukaryotes

38.4K
The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
38.4K
Overview of Archaea01:29

Overview of Archaea

979
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
979
Diversity of Archaea I01:30

Diversity of Archaea I

628
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
628
Diversity of Archaea II01:24

Diversity of Archaea II

524
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
524
Viruses of Archaea01:29

Viruses of Archaea

502
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
502
Diversity of Archaea IV01:29

Diversity of Archaea IV

455
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
455

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assembly and Reactions of Artificial Metalloenzymes in <i>Streptomyces albus</i>.

Journal of the American Chemical Society·2026
Same author

Counting strands in outer membrane β-barrels.

Biophysical journal·2026
Same author

Engineering electron conduits in bacteria for selective biointerfacing and enhanced energy transfer.

iScience·2026
Same author

Two-stage process and strain engineering for continuous bioconversion of CO<sub>2</sub> to butanol.

Bioresource technology·2025
Same author

Engineering Noncanonical Cofactors To Expand Cellular Functions.

ACS synthetic biology·2025
Same author

CO<sub>2</sub> upgrading into bioproducts using a two-step abiotic-biotic system.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Mammalian Respiratory Chain Complex Assemblies and Their Links to Mitochondria Stress-Induced Human Diseases.

Advances in experimental medicine and biology·2026
Same journal

Enzyme Assemblies in Nucleotide Metabolism: Structure, Regulation, and Disease Implications.

Advances in experimental medicine and biology·2026
Same journal

The Pyruvate Dehydrogenase Complex: A 90-Year-Old Enigma Shaping the Future of Structural Enzymology.

Advances in experimental medicine and biology·2026
Same journal

Regulation of the Anti-termination RNA Transcription Complex by Lon-Mediated Lambda N Degradation.

Advances in experimental medicine and biology·2026
Same journal

PCNA Macromolecular Complexes: PCNA Serves as a Molecular Hub Regulating Multiple Cellular Processes Inside and Outside of the Nucleus.

Advances in experimental medicine and biology·2026
Same journal

Dynamic Assemblies in Genome Maintenance.

Advances in experimental medicine and biology·2026
See all related articles

Related Experiment Video

Updated: Feb 2, 2026

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
07:20

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Published on: April 9, 2019

9.7K

Prefoldins in Archaea.

Samuel Lim1, Dominic J Glover2, Douglas S Clark3

  • 1Department of Chemical and Biological Engineering, University of California, Berkeley, CA, USA.

Advances in Experimental Medicine and Biology
|November 29, 2018
PubMed
Summary
This summary is machine-generated.

Archaeal prefoldins (PFDs) are versatile molecular chaperones that stabilize diverse proteins. Their unique structure and function are valuable for biotechnology and nanotechnology applications.

Keywords:
AggregationArchaeaChaperoneChaperoninCoiled-coilNanotechnologyPrefoldinProtein foldingSelf-assemblyThermostability

More Related Videos

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.1K
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

12.1K

Related Experiment Videos

Last Updated: Feb 2, 2026

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
07:20

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Published on: April 9, 2019

9.7K
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.1K
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

12.1K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Nanotechnology

Background:

  • Molecular chaperones, including prefoldins (PFDs), are crucial for protein folding in cells.
  • Archaeal PFDs possess a distinct jellyfish-like hexameric structure, differing from eukaryotic PFDs.
  • Archaeal PFDs play a vital role in chaperone systems, often compensating for the absence of Hsp70.

Purpose of the Study:

  • To explore the structural diversity and substrate-binding capabilities of archaeal prefoldins.
  • To highlight the biotechnological and nanotechnological applications of archaeal PFDs.

Main Methods:

  • Characterization of archaeal PFD structure, including hexameric, tetrameric, and filamentous forms.
  • Investigation of PFDs' interaction with nonnative proteins and subsequent transfer to chaperonins.
  • Exploration of PFDs' utility in biotechnology and nanotechnology.

Main Results:

  • Archaeal PFDs exhibit structural diversity, existing as various oligomers beyond hexamers.
  • PFDs effectively bind and stabilize a wide range of nonnative protein substrates.
  • A filament-forming PFD variant has been utilized for nanoscale architecture fabrication.

Conclusions:

  • Archaeal PFDs are adaptable molecular chaperones with broad substrate stabilization capabilities.
  • The unique structural and functional properties of archaeal PFDs offer significant potential in biotechnology and nanotechnology.
  • PFDs can be engineered for applications such as nanoscale material construction.