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Growth-Stimulating Effect of Human Platelets Stabilized with Silver Nanoparticles
M S Makarov1, M V Storozheva2, N V Borovkova2
1N. V. Sklifosovsky Research Institute of Emergency Medicine, Moscow Healthcare Department, Moscow, Russia. mcsimmc@yandex.ru.
Bulletin of Experimental Biology and Medicine
|November 30, 2018
Summary
Silver nanoparticle-stabilized platelets stimulate multipotent mesenchymal stromal cell growth and migration in collagen matrices. Higher doses of stabilized platelets inhibit proliferation and cause cell damage, impacting cell therapy applications.
Area of Science:
- Biomaterials Science
- Cell Biology
- Nanotechnology
Background:
- Multipotent mesenchymal stromal cells (MSCs) are crucial for tissue regeneration.
- Platelets play a role in wound healing and cell proliferation.
- Silver nanoparticles offer antimicrobial and potential bioactivity properties.
Purpose of the Study:
- To investigate the effect of silver nanoparticle-stabilized platelets on MSCs.
- To determine the dose-dependent response of MSCs to stabilized platelets.
- To evaluate the impact on MSC proliferation, migration, and structural integrity.
Main Methods:
- Culturing MSCs on collagen matrices.
- Incorporating varying concentrations of silver nanoparticle-stabilized platelets.
- Assessing cell proliferation using cell counting and viability assays.
- Evaluating cell migration and matrix colonization.
- Microscopic examination for structural integrity.
Main Results:
- Dose-dependent stimulation of MSC proliferation was observed with stabilized platelets (20-120 million per 10^5 MSCs) without structural damage.
- Stabilized platelets showed a more pronounced stimulation effect compared to non-stabilized platelets at equivalent doses.
- Higher concentrations (≥180 million) of stabilized platelets inhibited MSC proliferation and induced cell damage.
- Stabilized platelets enhanced MSC migration and colonization within the collagen matrix.
- Formed MSC monolayers remained viable for over 14 days without significant damage.
Conclusions:
- Silver nanoparticle-stabilized platelets can modulate MSC behavior in a dose-dependent manner.
- Optimized concentrations of stabilized platelets promote MSC proliferation and migration, suggesting therapeutic potential.
- Excessive concentrations of stabilized platelets can be cytotoxic, necessitating careful dose control in applications.
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