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A Protocol for Transcranial Photobiomodulation Therapy in Mice
Published on: November 18, 2018
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A Protocol for Transcranial Photobiomodulation Therapy in Mice.
Farzad Salehpour1, Luis De Taboada2, Paolo Cassano3
1Neurosciences Research Center, Tabriz University of Medical Sciences; ProNeuroLIGHT LLC.
Journal of Visualized Experiments : Jove
|December 4, 2018
Summary
Transcranial photobiomodulation therapy (PBMT) using a 660 nm laser improved spatial memory and hippocampal ATP levels in aged mice. This noninvasive approach shows promise for age-related cognitive decline.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Gerontology
Background:
- Age-related cognitive decline and neurodegenerative diseases impact brain function and memory.
- Noninvasive therapeutic approaches are needed to address these conditions.
- Photobiomodulation offers a potential strategy for enhancing brain bioenergetics.
Purpose of the Study:
- To describe a laboratory protocol for transcranial photobiomodulation therapy (PBMT) in aged mice.
- To evaluate the effects of PBMT on spatial learning, memory, and hippocampal energy metabolism.
- To assess the potential of PBMT as a therapeutic approach for age-related cognitive impairment.
Main Methods:
- Aged BALB/c mice (18 months old) were treated transcranially with a 660 nm laser daily for 2 weeks.
- Laser transmittance was measured to determine light penetration depth (approx. 1% at 1 mm).
- Spatial memory was assessed using the Barnes maze test; hippocampal ATP levels were measured as a bioenergetics index.
Main Results:
- Laser-treated aged mice showed enhanced spatial memory performance compared to controls.
- Biochemical analysis revealed increased hippocampal ATP levels post-treatment.
- The results suggest improved hippocampal energy metabolism induced by red laser treatment.
Conclusions:
- Transcranial photobiomodulation (PBMT) effectively enhanced spatial memory and hippocampal ATP levels in aged mice.
- PBMT is a safe, cost-effective, and noninvasive modality with therapeutic potential for age-related cognitive impairment.
- The described protocol is adaptable for translational neuroscience research in various animal models.
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