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Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
Published on: January 22, 2014
[TIME SINCE DEATH ESTIMATION BY USING THE METHOD OF POLYCRYSTALLINE CEREBROSPINAL FLUID FILMS IMAGES MUTUAL
M Garazdiuk1, V Bachinskyi1, O Vanchuliak1
1Higher State Educational Establishment of Ukraine "Bukovinian State Medical University", Chernivtsi, Ukraine.
Abstract:
Objective - to develop a method of two-dimensional Stokes-polarimetric spatial-frequency mapping of small-scale components of cerebrospinal fluid to improve the accuracy of post-mortem interval estimation. The object of the study was polycrystalline cerebrospinal fluid films taken from 69 corpses (the main study group) and 20 healthy volunteers (comparison group). For each sample, the coordinate distribution of the values of the complex degree of mutual polarization was determined in the optical arrangement of the Stokes polarimeter. The value of the statistical moments of 1 - 4 orders with further statistical processing was calculated. Time dependences of the variation of the value of the most sensitive statistical moments were built to achiev of values stabilization. The interval and the accuracy of the post-mortem interval were estimated by generalizing of the time dependences of the third and fourth order statistical moments of the polarization maps obtained by the two-dimensional mapping of the values distributions of the complex degree of mutual polarization of the small-scale component of polycrystalline networks of cerebrospinal fluid films. An interval of 10 h and the accuracy of post-mortem interval estimation ΔT = ± 12.5 min was established.
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