Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Protein Complex Assembly02:41

Protein Complex Assembly

2.6K
2.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.9K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.9K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.1K
2.1K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
Components of Stress01:23

Components of Stress

536
Stress analysis under multiple loading conditions is intricate, necessitating a comprehensive grasp of normal and shearing stresses. Consider a small cube at point O, subjected to stress on all six faces, visible or not. Normal stress components σx, σy, σz act perpendicularly to the x, y, and z axes. Shearing stress components τxy and τxz are exerted on faces perpendicular to these axes.
Interestingly, the hidden cube faces also experience these stresses, equal and...
536

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera).

Molecular biology and evolution·2026
Same author

Co-culturing hiPSC-cardiomyocytes and cardiac fibroblasts enhances engineered heart tissue structure and function.

Stem cells translational medicine·2026
Same author

Enzymatic Deacetylation as a Selective Strategy for <i>O</i>-GlcNAc Identification.

Analytical chemistry·2026
Same author

Liposomal lipid nanoparticles containing dihydrosphingomyelin exhibit improved stability and extended hepatic and extrahepatic transfection.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

European foulbrood disease and protein supplementation in honey bee (<i>Apis mellifera</i> L.) colonies pollinating highbush blueberries in British Columbia.

PeerJ·2026
Same author

Ribosome biogenesis mediates the translational increase of nonoptimal codon transcripts during IFN-β stimulation.

Canadian journal of microbiology·2026
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Related Experiment Video

Updated: Feb 1, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

28.1K

Dynamics of protein complex components.

David G Rattray1, Leonard J Foster1

  • 1University of British Columbia, Michael Smith Laboratories, 2329 West Mall, Vancouver, BC V6T 1Z4, Canada.

Current Opinion in Chemical Biology
|December 12, 2018
PubMed
Summary
This summary is machine-generated.

Identifying protein-protein interactions (PPIs) is crucial for understanding cellular processes. This review summarizes techniques for identifying PPIs, addressing challenges like complex arrangements and transient interactions.

More Related Videos

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.9K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.6K

Related Experiment Videos

Last Updated: Feb 1, 2026

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
13:34

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

28.1K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.9K
Identification of Post-translational Modifications of Plant Protein Complexes
10:07

Identification of Post-translational Modifications of Plant Protein Complexes

Published on: February 22, 2014

24.6K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Understanding cellular mechanisms requires identifying protein-protein interactions (PPIs).
  • Proteins can form complexes and participate in multiple interactions, complicating identification.
  • Interaction stability, ranging from stable to transient, adds complexity to PPI studies.

Purpose of the Study:

  • To review current techniques for identifying protein-protein interactions.
  • To highlight recent advancements in PPI identification methodologies.

Main Methods:

  • This review summarizes various experimental and computational approaches used for PPI detection.
  • Focus is placed on methods addressing the complexities of protein complex formation and interaction dynamics.

Main Results:

  • A range of techniques exist to identify PPIs, each with specific strengths and limitations.
  • Recent developments aim to improve the accuracy and scope of PPI identification.

Conclusions:

  • Accurate identification of PPIs and their dynamics is essential for deciphering cellular functions.
  • Continued development of novel techniques is necessary to overcome existing challenges in PPI research.