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Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

Cis-regulatory Sequences

Cis-regulatory Sequences

Sequences

Sequences

Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Arithmetic Sequences

Arithmetic Sequences

An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...

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Related Experiment Video

Updated: Jan 31, 2026

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

Single-Cell Applications of Next-Generation Sequencing.

Naishitha Anaparthy¹, Yu-Jui Ho², Luciano Martelotto³

¹Department of Molecular and Cellular Biology, Stony Brook University, Stony Brook, New York 11794.

Cold Spring Harbor Perspectives in Medicine

|January 9, 2019

Summary

This summary is machine-generated.

Single-cell sequencing, a powerful tool from next-generation sequencing (NGS), analyzes individual cell molecular profiles. This technology is revolutionizing basic and clinical research in fields like developmental genetics and cancer.

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Last Updated: Jan 31, 2026

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing

Published on: October 11, 2021

Detection of Copy Number Alterations Using Single Cell Sequencing

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Published on: February 10, 2023

Area of Science:

Molecular Biology
Genomics
Developmental Biology

Background:

The single cell is the fundamental unit of life.
Understanding cellular heterogeneity in various biological systems is crucial.
Next-generation sequencing (NGS) enables large-scale molecular profiling of individual cells.

Purpose of the Study:

To provide a non-expert overview of single-cell sequencing.
To highlight the impact of single-cell sequencing on biological and clinical research.

Main Methods:

Leveraging next-generation sequencing (NGS) technologies.
Assaying hundreds to millions of cells in parallel.
Generating molecular profiles (RNA and DNA) for individual cells.

Main Results:

Single-cell sequencing offers an inexpensive method for detailed cellular analysis.
It provides mathematical tractability for complex biological data.
Enables deep insights into cellular function within complex environments.

Conclusions:

Single-cell sequencing is transforming the study of cell populations.
Its applications span embryology, developmental genetics, and cancer research.
Facilitates advancements in both fundamental biological understanding and clinical applications.