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    Area of Science:

    • Biomedical optics
    • Microscopy techniques
    • Image processing

    Background:

    • Full-field swept-source optical coherence microscopy (OCM) offers 3D imaging capabilities.
    • Improving transverse resolution in OCM is crucial for detailed sample analysis.
    • Numerical focusing presents a potential method for enhancing OCM image quality.

    Purpose of the Study:

    • To investigate the combination of structured illumination and numerical post-processing for transverse resolution enhancement in OCM.
    • To demonstrate effective numerically focused 3D imaging in full-field OCM.
    • To evaluate the feasibility of this combined approach for imaging biological samples.

    Main Methods:

    • Utilizing structured illumination with Fourier domain OCM.
    • Implementing specific numerical post-processing algorithms for image reconstruction.
    • Imaging a resolution test target in and out of optical focus.
    • Imaging a 3D phantom and a biological sample.

    Main Results:

    • Demonstrated transverse resolution improvement of the OCM coherence signal.
    • Achieved effective numerically focused 3D imaging in both focus and defocus regions.
    • Successfully imaged a 3D phantom and a biological sample with high transverse resolution.

    Conclusions:

    • The combination of structured illumination and numerical focusing is feasible for Fourier domain OCM.
    • This approach significantly enhances transverse resolution and enables effective 3D imaging.
    • The technique shows promise for advanced microscopic imaging applications.