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Updated: Jan 30, 2026

Author Spotlight: Asymmetric Field Flow Fractionation for Bioreactor Integration
Published on: February 2, 2024
Single-step assembly of asymmetric vesicles.
Laura R Arriaga1, Yuting Huang, Shin-Hyun Kim
1School of Engineering and Applied Science and Department of Physics, Harvard University, 02138 Cambridge, MA, USA. weitz@seas.harvard.edu.
Researchers developed a new method using triple emulsion drops to create asymmetric vesicles, which mimic cell membranes. This breakthrough overcomes fabrication challenges, enabling advanced applications in drug delivery and cosmetics.
Area of Science:
- Materials Science
- Biomimetic Chemistry
Background:
- Asymmetric vesicles, with unevenly distributed amphiphiles, are crucial for mimicking biological membranes.
- Their asymmetry enhances applications in drug delivery and cosmetics.
- Conventional amphiphile self-assembly typically results in symmetric vesicles, posing a fabrication challenge.
Purpose of the Study:
- To develop a novel method for fabricating asymmetric vesicles.
- To control the degree of asymmetry in vesicle membranes.
Main Methods:
- Utilized water-in-oil-in-oil-in-water triple emulsion drops.
- Employed capillary microfluidics for precise fabrication of monodisperse drops.
- Controlled amphiphile diffusion and adsorption at oil/water interfaces within the emulsion.
Main Results:
- Achieved asymmetric vesicle formation with up to 70% asymmetry.
- Demonstrated efficient encapsulation of various ingredients within the vesicles.
- Fabrication process achieved high production rates (300 Hz).
Conclusions:
- Triple emulsion drops provide a viable strategy for directed assembly of asymmetric vesicles.
- The method offers precise control over vesicle asymmetry and composition.
- These asymmetric vesicles hold significant potential for artificial cells, bioreactors, and advanced delivery systems.
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