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Updated: Jan 30, 2026

Adjunctive Diode Laser Therapy and Probiotic Lactobacillus Therapy in the Treatment of Periodontitis and Peri-Implant Disease
Published on: May 9, 2022
[EXPERIMENTAL RATIONALE OF ENDODONTIC THERAPY OF CHRONIC FORMS OF PULPITIS- AND PERIODONTITIS USING PHOTO-ACTIVATED
This study shows that combining ultrasound treatment with photo-activated disinfection effectively suppresses oral bacteria, including those found in chronic pulpitis and periodontitis.
Area of Science:
- Microbiology
- Dental Research
- Biotechnology
Background:
- Chronic pulpitis and periodontitis are often associated with complex oral microflora.
- Biofilms in dentinal tubules pose a significant challenge in treating these conditions.
Purpose of the Study:
- To evaluate the in vitro efficacy of a combined ultrasound treatment and photo-activated disinfection approach.
- To assess the impact of this combined therapy on anaerobic and microaerophilic microorganisms relevant to endodontic infections.
Main Methods:
- Bacteriologic analysis of root canal microflora, including obligate anaerobes and microaerophilic streptococci.
- In vitro evaluation using a bioreactor to test the combined therapy's effect on root canal microflora.
- Scanning electron microscopy to study root canal biofilms in patients with chronic pulpitis and periodontitis.
Main Results:
- Significant microbial biofilm presence was observed in dentinal tubule openings.
- The combined ultrasound and photo-activated disinfection significantly suppressed the growth of oral bacterial populations, including Porphyromonas gingivalis and Fusobacterium nucleatum.
- Growth curves demonstrated a marked reduction in bacterial proliferation under the combined treatment compared to controls.
Conclusions:
- The combined application of ultrasound treatment and photo-activated disinfection demonstrates significant antimicrobial efficacy.
- This approach shows promise for managing microbial challenges in chronic endodontic infections like pulpitis and periodontitis.
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