[ANALYSIS OF EFFECTIVENESS OF USINGJIgY FROM CHICKEN IN SANDWICH METHOD OF HBsAg TESTING]

Study antigen-binding ability of polyclonal antibodies (PCA) of chicken compared with monoclonal -antibodies (MCA) of mice in the model of interaction with HBsAg.

Mice MCA 18C8 and MKA F3/F4 (IgG) were used, effective in enzyme immunoassay sandwich method of HBsAg determination (with a minimal detection dose of 0.017 ng/ml), and affinity purified anti-HBsAg PCA of chicken (IgY), obtained from 2 immunized birds (PCA No. 1 and PCA No. 2). The ability of antibodies to bind HBsAg was evaluated by analytical sensitivity (slope of binding curve) of solid-phase enzyme immunoassay system using mice MCA and chicken PCA.

PCA No. 2 has provided a statistically significant 40% increase of analytical sensitivity, compared with <

A lower flexibility of chicken PCA was assumed to be able to impede bivalent interaction in sandwich method, resulting in formation of less stable imminne complexes. Without Challenging value of IgY for the creation of immunochemical diagnostic methods, these facts and assumptions indicate a necessity of a deeper elucidation of the best ar- eas of their application.