Related Experiment Video
Updated: Jan 29, 2026

Shock Wave Application to Cell Cultures
Published on: April 8, 2014
Secreted heat shock proteins in sunflower suspension cell cultures
1Istituto di Ricerca sulle Biotecnologie Agroalimentari C.N.R., Via Monteroni, I-73100 Lecce, Italy Fax no.: +39-832-420000, , , , , , IT.
Abstract:
Sunflower suspension cell cultures were subjected to different heat treatments and the electrophoretic patterns of heat-induced endocellular and secreted proteins were analyzed. In response to heat shock (3 h at 40°C), sunflower cells synthesized new polypeptides and secreted them into the medium, while the synthesis of other polypeptides was suppressed. Two major polypeptides of about 50 and 32 kDa were strongly induced. The two-dimensional electrophoretic analysis showed that the 32-kDa band is composed of at least four different polypeptides. Western blotting hybridizations of secreted proteins with various lectins were performed. The 32-kDa band gave a positive signal with concanavalin A.
More Related Videos
Related Concept Videos
Bacterial Translocation and Protein Secretion
Responses to Heat and Cold Stress
Cells and Secretions of the Pancreas
Exocrine function is carried out by acinar cells, organized into clusters known as acini. These cells contribute to digestion by releasing substantial quantities of enzyme-rich, alkaline digestive juices.
Concurrently, the dispersed clusters of endocrine cells throughout the...
Gram-negative Bacterial Protein Secretion Systems
Shock Waves
When the source's speed approaches the speed of sound, constructive interference between successive wavefronts emitted by the source occurs immediately behind it. Initially, scientists believed that this constructive interference would result in such high...
Specific Heat
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...

