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Updated: Jan 29, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
A leucine zipper-based peptide hybrid delivers functional Nanog protein inside the cell nucleus
Yoshiyuki Hakata1, Hiroyuki Michiue2, Takashi Ohtsuki3
1Department of Immunology, Faculty of Medicine, Kindai University, 377-2 Ohno-Higashi, Sayama, Osaka 589-8511, Japan.
Abstract:
We synthesized a pair of compounds containing leucine zipper peptides to deliver protein cargo into cells. One is a cell-penetrating peptide (CPP) with Lz(E), a leucine zipper peptide containing negatively charged amino acids, and the other is a Nanog protein with Lz(K), a leucine zipper peptide containing positively charged amino acids. When cells were treated with these equimolar mixtures, Nanog-Lz(K) hybridized with Lz(E)-CPP was successfully delivered into the cells. Furthermore, Nanog-Lz(K) exerted its proper function after nuclear transport.
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