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Drug with Neuroprotective Properties Noopept Does Not Stimulate Cell Proliferation

L F Zainullina1, T V Ivanova2, R U Ostrovskaya1

  • 1V. V. Zakusov Research Institute of Pharmacology, Moscow, Russia.

Bulletin of Experimental Biology and Medicine
|February 22, 2019
PubMed

Insights

Noopept, a nootropic drug, was investigated for its potential to stimulate cell growth. Studies on HEK293 and SH-SY5Y cells found that Noopept does not affect cell cycle progression or the proliferation marker Ki-67, indicating it does not promote cell proliferation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Cell Biology

Background:

  • Noopept (N-phenylacetyl-L-prolylglycine ethyl ester) is a nootropic agent with a known multifactorial mechanism of action.
  • Its effects include enhancing neurotrophin NGF and BDNF expression and increasing HIF-1 activity.
  • The potential mitogenic effects of Noopept warrant investigation.

Purpose of the Study:

  • To evaluate the impact of Noopept on cell proliferation.
  • To assess the effects of Noopept on cell cycle parameters.
  • To determine the influence of Noopept on the proliferation marker Ki-67.

Main Methods:

  • Experiments were conducted using HEK293 and SH-SY5Y cell lines.
  • Cell cycle distribution across G1, S, and G2 phases was analyzed.
  • The relative level of the proliferation marker Ki-67 was quantified.

Main Results:

  • Noopept did not alter the distribution of cells in the G1, S, or G2 phases of the cell cycle.
  • The relative level of the proliferation marker Ki-67 remained unaffected by Noopept treatment.
  • No significant changes in cell cycle parameters or Ki-67 levels were observed.

Conclusions:

  • Noopept does not appear to stimulate cell growth or proliferation.
  • The drug's mechanism of action does not involve the direct stimulation of cell division.
  • These findings contribute to understanding the safety profile and cellular effects of Noopept.
Keywords:
Ki-67NoopeptPro-Gly substituted dipeptidescell cycleproliferation

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