Related Experiment Video
Updated: Jan 28, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii
Sarah E Anderson1,2, Philip N Rather3
1Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA.
Abstract:
Many strains of Acinetobacter baumannii, including the highly virulent strain AB5075, undergo a high-frequency switch that results in two cell types that are distinguished by their opaque or translucent colony opacities when viewed by oblique lighting. Opaque (VIR-O) and translucent (AV-T) colonies exhibit multiple phenotypic differences, including virulence. Here we describe how to distinguish between VIR-O and AV-T colony variants and how to generate highly pure stocks of each variant. We also describe methods for measuring opacity switching frequencies of cells grown on agar plates and in liquid cultures.
More Related Videos
09:29In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging
Published on: April 28, 2017
07:41Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Related Concept Videos
Histone Variants at the Centromere
What is Variation?
The range, standard deviation, standard error, and variance are the different measures of variation.
Range: The range is the difference between its maximum and...
Variation
When independent and dependent variables are plotted on a scatter plot, the slope of a line is a value that describes the rate of change between the two...
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Variation: Normal Distribution, Range, and Standard Deviation
Variation of Atmospheric Pressure
Assuming the air temperature is constant at a given altitude and that the ideal gas law of thermodynamics describes the atmosphere to a good approximation, one can find the variation of atmospheric pressure with height.
Let p(y) be the atmospheric pressure at...