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Expression of Snail and E-cadherin in Drug-resistant MCF-7/ADM Breast Cancer Cell Strains

Fenglin Cai1, Hualin Xiao2, Yuting Sun2

  • 1Department of General Surgery, The Affiliated Cancer Hospital of Nanjing Medical University and Jiangsu Cancer Hospital, and Jiangsu Institute of Cancer Research, 210009, China.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP
|March 3, 2019
PubMed
Abstract

Insights

Drug-resistant breast cancer cells show increased Snail and decreased E-cadherin expression. This finding offers new avenues for targeted therapy in adriamycin-resistant breast cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Breast cancer remains a significant global health challenge.
  • Adriamycin resistance is a major obstacle in effective breast cancer treatment.
  • Understanding the molecular mechanisms of drug resistance is crucial for developing new therapies.

Purpose of the Study:

  • To investigate the differential expression of Snail and E-cadherin in adriamycin-resistant (MCF-7/ADM) versus non-resistant (MCF-7) human breast cancer cells.
  • To analyze the role of these key transcription factors in the development of adriamycin resistance.

Main Methods:

  • Utilized real-time quantitative PCR to assess mRNA expression levels of Snail and E-cadherin.
  • Employed Western blot analysis to determine the protein expression levels of Snail and E-cadherin.
  • Compared expression patterns in normal breast cells, MCF-7/ADM cells, and MCF-7 cells.

Main Results:

  • Significantly higher expression of Snail mRNA and protein was observed in MCF-7/ADM cells compared to normal and MCF-7 cells (p<0.001).
  • Significantly lower expression of E-cadherin mRNA and protein was detected in MCF-7/ADM cells compared to normal and MCF-7 cells (p<0.001).

Conclusions:

  • Adriamycin-resistant human breast cancer MCF-7/ADM cells exhibit elevated Snail and diminished E-cadherin expression.
  • These findings suggest Snail and E-cadherin as potential therapeutic targets for overcoming adriamycin resistance in breast cancer.
  • Provides valuable insights for clinical breast cancer therapy and prognosis evaluation.

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