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Updated: Jan 28, 2026

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay
Published on: May 12, 2023
THE SPECIFIC FEATURES OF CELL DEATH OF CIRCULATING NEUTROPHILS IN A SETTING OF EXPERIMENTALLY INDUCED CRUSH SYNDROME
T Pylypchuk1, D Delibashvili1, R Usynskyi1
1I. Horbachevsky Ternopil State Medical University, Ukraine; Tbilisi State Medical University, Georgia.
Abstract:
Although the overall incidence of trauma-induced crush syndrome (CS) is as low as 0.1 per 10,000 of population, which makes it one of the least common traumatic injuries, the hazard of CS lies in the high incidence of hemodynamic and metabolic complications, which call for an early diagnosis of this syndrome. This paper reports the specific features of cell death of neutrophils in rat blood as they changed with time using the model of endogenous toxemia formed in a setting of crush syndrome. To assess apoptosis/necrosis in neutrophils ANNEXIN V FITC assay kit (manufactured by Beckmann Coulter, USA) was used. The results obtained in this study suggest an increase in apoptotic and necrotic processes in a setting of CS from the first day of the experiment through the end of observation. Also we have found a significant impact of reactive oxygen species generation on neutrophils death in a setting of CS. However, other mechanisms to launch apoptosis/necrosis in case of experimental crush syndrome should not be neglected.
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