[PRODUCTION OF REACTIVE OXYGEN SPECIES AND DEVELOPMENT OF APOPTOSIS IN BLOOD LEUKOCYTES IN EXPERIMENTAL...

The aim of the study is to investigate the processes of apoptosis and the level of formation of reactive oxygen species (ROS) in blood cells of BALB/c female mice in experimental antiphospholipid syndrome (APS). The content of living, apoptotic and necrotic cells in blood leukocytes was evaluated. Apoptosis in cells was detected using Annexin V conjugated to GFP and propidium iodide. ROS in blood leukocytes were detected using 2',7'-dichlorofluorescein diacetate. The redistribution between the leukocytes populations was assessed by the magnitude of direct (FS) and lateral (SS) light scattering on the flow cytofluorimeter. It was established that the APS reduced the viability of blood leukocytes compare to the control. It is likely that their death is due partly to the activation of apoptosis. In the animals with APS a redistribution between the two main types of leukocytes (granulocytes and agranulocytes) was found out. An increase in the number of granulocytes in the blood was evidenced in cases of APS. It was established that the basal level of ROS production in granulocytes reduced by 27% and in agranulocytes - by 19% compare to the control. Thus, taking into account the attained results it can be argued that in the pathobiochemical mechanisms of APS development the enhanced activation of apoptosis and deficient formation of ROS is significant.