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Updated: Jan 28, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
Published on: December 5, 2018
Flow Cytometry-Based Cell Type-Specific Assessment of Target Regulation by Pulmonary siRNA Delivery
Olivia M Merkel1,2, Leigh M Marsh3,4, Holger Garn3,5
1Department of Pharmacy, Ludwig-Maximilians-Universität München, Munich, Germany. Olivia.Merkel@cup.uni-muenchen.de.
Abstract:
Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected has so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein (EGFP) expressing mice for the differentiation of transfected lung cell populations based on their antigen expression.
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