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Naringin (4',5,7-Trihydroxyflavanone 7-Rhamnoglucoside) Attenuates β-Cell Dysfunction in Diabetic Rats through
Manickam Subramanian1, Balaji Thotakura2, Swathi Priyadarshini Chandra Sekaran2
1Department of Anatomy, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Chennai, India, gandhiayu@hotmail.com.
Cells, Tissues, Organs
|March 19, 2019
Summary
Naringin, an antioxidant flavanone, effectively treats diabetes by upregulating pancreatic duodenal homeobox-1 (PDX-1) and insulin gene expression. This enhances insulin secretion and restores pancreatic beta-cell function in diabetic rats.
Area of Science:
- Endocrinology
- Molecular Biology
- Pharmacology
Background:
- Pancreatic duodenal homeobox-1 (PDX-1) is crucial for insulin gene expression, insulin secretion, and beta-cell mass maintenance.
- Naringin, a flavanone with antioxidant properties, exhibits potential antidiabetic effects.
Purpose of the Study:
- To investigate the effect of naringin on the beta-cell-specific transcription factor PDX-1 in a rat model of diabetes.
- To evaluate naringin's impact on insulin regulation and pancreatic beta-cell health.
Main Methods:
- Diabetes was induced in male rats using streptozotocin.
- Rats were orally administered naringin (100 mg/kg) for 4 and 8 weeks.
- Assessed serum insulin, Pdx-1 and Insulin gene/protein expression, and pancreatic islet/beta-cell histology.
Main Results:
- Naringin treatment reduced pancreatic leukocytic infiltration and improved beta-cell secretory granules.
- Significant increases in Pdx-1 and Insulin mRNA and PDX-1 protein expression were observed in naringin-treated rats.
- Higher serum insulin levels and more pronounced improvements were noted after 8 weeks of naringin treatment.
Conclusions:
- Naringin demonstrates efficacy as an antidiabetic agent.
- Naringin upregulates PDX-1 gene and protein expression, thereby enhancing insulin gene expression and secretion.
- Naringin effectively improves pancreatic beta-cell function in diabetic rats.