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Updated: Jan 27, 2026
08:35
Production and Titering of Recombinant Adeno-associated Viral Vectors
Published on: November 27, 2011
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Recombinant Viral Vectors as Neuroscience Tools.
Shih-Heng Chen1,2, Juhee Haam2, Mitzie Walker1,2
1Viral Vector Core, National Institute of Environmental Health Sciences, NIH/DHHS, Research Triangle Park, North Carolina.
Current Protocols in Neuroscience
|March 23, 2019
Summary
This review explores viral vectors for gene delivery in the brain. It details adeno-associated viruses, retro/lentiviruses, and rabies viruses for neurobiology research.
Area of Science:
- Neurobiology
- Molecular Biology
- Genetics
Background:
- Recombinant viruses are effective for in vivo gene delivery.
- Viral vectors are crucial tools in neurobiology for brain research.
- Different viral vectors have unique strengths and weaknesses.
Purpose of the Study:
- To provide an overview of viral recombinant technology.
- To compare common viral vectors used in neurobiology.
- To guide the selection of appropriate viral vectors for research.
Main Methods:
- Review of viral recombinant technology.
- Analysis of viral structure and tropism.
- Comparison of adeno-associated viruses, retro/lentiviruses, and rabies viruses.
Main Results:
- Adeno-associated viruses, retro/lentiviruses, and rabies viruses are key vectors.
- Understanding serotypes and pseudotypes is essential for vector selection.
- Each vector type has specific applications in neuroscience.
Conclusions:
- Selecting the right viral vector is critical for successful gene delivery in the brain.
- This article serves as a guide for neurobiologists.
- Knowledge of viral properties aids in experimental design.
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