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Updated: Jan 26, 2026

Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
Comparative Thermal Resistance of Human and Simian Rotaviruses Assayed on Cells Grown in a Serum-Free Medium
Dale J VAN Donsel1, James T Peeler1, Edward P Larkin1
1Virology Branch, Division of Microbiology, Center for Food Safety and Applied Nutrition, Food and Drug Administration, Cincinnati, Ohio 45226.
Abstract:
Thermal resistance of the Wa strain of human rotavirus was compared with that of SA-11 simian rotavirus in a plaque assay with MA-104 cells grown in conventional cell culture medium supplemented with filtrate from reconstituted and acid-precipitated nonfat dry milk. Both viruses produced two-component survival curves at 56 and 60°C. Average D-values (min) for the Wa human virus were 306.3 at 56°C, 47.8 at 60°C, 12.3 at 62.8°C and 2.4 at 65°C, with a z-value of 4.33°C. For the SA-11 simian virus, the average D-values were 890.5 at 56°C, with a z-value of 4.35°C. These values indicate that low levels of rotavirus should be inactivated by pasteurization processes.
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