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Related Concept Videos

pre-mRNA Processing02:01

pre-mRNA Processing

57.3K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
57.3K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.7K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Transcription01:10

Transcription

155.9K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.9K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.6K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.6K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.6K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

8.1K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
8.1K

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Related Experiment Video

Updated: Jan 26, 2026

Measuring the Kinetics of mRNA Transcription in Single Living Cells
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Measuring the Kinetics of mRNA Transcription in Single Living Cells

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Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System.

Eleen Y Shum1, Elisabeth M Walczak2, Christina Chang2

  • 1BD Biosciences, San Jose, CA, USA. Eleen.Shum@bd.com.

Advances in Experimental Medicine and Biology
|April 11, 2019
PubMed
Summary

The BD Rhapsody system offers high-throughput single-cell analysis for nucleic acid capture. This platform enables whole transcriptome and targeted RNA sequencing for detailed gene expression profiling.

Keywords:
AntibodyBarcoded beadsMagnetic beadsMicrowell arrayMolecular barcodingNext-generation sequencingSingle cellsTargeted RNA-seqTranscriptomeUMIUnique molecular identifiers

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Single Cell Analysis Of Transcriptionally Active Alleles By Single Molecule FISH

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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
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Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • Single-cell analysis is crucial for understanding cellular heterogeneity.
  • High-throughput methods are needed to profile gene expression at the single-cell level.
  • Existing platforms may have limitations in sensitivity or scope.

Purpose of the Study:

  • To describe the BD Rhapsody Single-Cell Analysis System.
  • To highlight its capabilities for nucleic acid capture and library generation.
  • To showcase its utility in advanced single-cell research.

Main Methods:

  • Utilizes a cartridge-based workflow for nucleic acid capture from single cells.
  • Employs a multitier barcoding system for high-throughput processing.
  • Supports the generation of various next-generation sequencing (NGS) libraries.

Main Results:

  • Enables whole transcriptome analysis for discovery biology.
  • Facilitates targeted RNA analysis for sensitive transcript detection.
  • Integrates with assays like BD™ AbSeq for simultaneous mRNA and protein profiling.

Conclusions:

  • The BD Rhapsody system provides a versatile platform for ultra-high resolution single-cell analysis.
  • It supports diverse applications, from basic research to sensitive diagnostic profiling.
  • The system advances the field of single-cell genomics and transcriptomics.