Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System

Area of Science:

• Genomics
• Molecular Biology
• Biotechnology

Background:

• Single-cell analysis is crucial for understanding cellular heterogeneity.
• High-throughput methods are needed to profile gene expression at the single-cell level.
• Existing platforms may have limitations in sensitivity or scope.

Purpose of the Study:

• To describe the BD Rhapsody Single-Cell Analysis System.
• To highlight its capabilities for nucleic acid capture and library generation.
• To showcase its utility in advanced single-cell research.

Main Methods:

• Utilizes a cartridge-based workflow for nucleic acid capture from single cells.
• Employs a multitier barcoding system for high-throughput processing.
• Supports the generation of various next-generation sequencing (NGS) libraries.

Main Results:

• Enables whole transcriptome analysis for discovery biology.
• Facilitates targeted RNA analysis for sensitive transcript detection.
• Integrates with assays like BD™ AbSeq for simultaneous mRNA and protein profiling.

Conclusions:

• The BD Rhapsody system provides a versatile platform for ultra-high resolution single-cell analysis.
• It supports diverse applications, from basic research to sensitive diagnostic profiling.
• The system advances the field of single-cell genomics and transcriptomics.