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Visualizing Early Infection Sites of Rice Blast Disease Magnaporthe oryzae on Barley Hordeum vulgare Using a Basic Microscope and a Smartphone
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Osa-miR167d facilitates infection of Magnaporthe oryzae in rice

Zhi-Xue Zhao1, Qin Feng1, Xiao-Long Cao1

  • 1Rice Research Institute and Key Lab for Major Crop Diseases, Sichuan Agricultural University, Chengdu, 611130, China.

Journal of Integrative Plant Biology
|April 20, 2019
PubMed

Insights

Osa-miR167d microRNA negatively regulates rice immunity against rice blast disease by suppressing its target gene ARF12. This finding offers a potential strategy for enhancing crop resistance to fungal pathogens.

Area of Science:

  • Plant Molecular Biology
  • Plant Pathology
  • Agricultural Science

Background:

  • MicroRNAs (miRNAs) are crucial regulators in plant development and stress responses.
  • Rice blast disease, caused by Magnaporthe oryzae, poses a significant threat to global rice production.
  • Understanding miRNA-mediated immunity in rice is vital for developing disease-resistant crop varieties.

Purpose of the Study:

  • To investigate the role of Osa-miR167d in rice immunity against Magnaporthe oryzae.
  • To elucidate the regulatory mechanism of Osa-miR167d in rice blast disease resistance.

Main Methods:

  • Expression analysis of Osa-miR167d in rice accessions with varying resistance levels post-inoculation.
  • Generation and analysis of transgenic rice lines overexpressing Osa-miR167d or its target mimic.
  • Gene knockout analysis of the target gene ARF12.

Main Results:

  • Osa-miR167d expression was suppressed in resistant rice and increased in susceptible rice upon M. oryzae infection.
  • Overexpression of Osa-miR167d led to increased susceptibility to rice blast, while target mimicry enhanced resistance.
  • Knockout of ARF12 resulted in hyper-susceptibility to M. oryzae.

Conclusions:

  • Osa-miR167d acts as a negative regulator of rice immunity against M. oryzae by downregulating ARF12.
  • The Osa-miR167d-ARF12 regulatory module is a potential target for improving rice blast disease resistance.

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