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Updated: Jan 25, 2026

Analysis of Oxidative Stress in Zebrafish Embryos
Published on: July 7, 2014
Effect of Chitosan-(Poly)Nitroxides on Normal and Tumor Cells under Conditions of Induced Oxidative Stress
V A Mumyatova1,2,3, A A Balakina4,5,6, V D Sen'4,5
1Institute of Problems Chemical Physics, Russian Academy of Sciences, Chernogolovka, Moscow region, Russia. vmumyatova@bk.ru.
Abstract:
The cytotoxicity and antioxidant effects of chitosan-(poly)nitoxides of different molecular weights containing a nitroxide radical of the piperidine structure were studied on tumor (HeLa, A172, and HepG2) and normal (Vero) cell lines. The chitosan-(poly)nitroxides exhibited low cytotoxicity. Under conditions of oxidative stress induced with tert-butyl hydroperoxide, the most pronounced decrease in ROS levels in the presence of chitosan-(poly)nitroxides was observed in normal cells. In cell homogenates, the decrease in malondialdehyde levels was observed only in the presence of low-molecular-weight chitosan-(poly)nitroxide irrespective of the cell line. Our data demonstrate that the cell-specific antioxidant properties of chitosan-(poly)nitroxides are related to their penetration into cells and interaction with intracellular membranes.
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