Related Experiment Video
Updated: Jan 24, 2026

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
Published on: November 29, 2013
Characterization of Hydroxy and Hydroperoxy Polyunsaturated Fatty Acids by Mass Spectrometry
Priscilla Bento Matos Cruz Derogis1,2, Adriano B Chaves-Fillho1, Sayuri Miyamoto3
1Departamento de Bioquímica, Instituto de Química - Universidade de São Paulo, São Paulo, SP, Brazil.
Abstract:
Lipids containing unsaturated doubles bonds are oxidized by enzymatic and non-enzymatic mechanisms yielding hydroperoxides and hydroxides as primary products.This process has been implicated in physiological and pathological mechanisms. Thus, precise characterization and quantification of lipid oxidation products in biological samples can provide important mechanistic insights. In this context, the use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) to characterize the primary products of polyunsaturated fatty acids, like hydroxides and hydroperoxides, is a sensitive, specific and versatile tool. Here we will focus on the characterization and specific analysis of hydroxy and hydroperoxy regioisomers of linoleic acid, arachidonic acid, eicosapentaenoic acid and docosahexaenoic acid by LC-MS/MS.
Related Concept Videos
Mass Spectrometry: Carboxylic Acid, Ester, and Amide Fragmentation
For example, the...
Overview of Fatty Acid Metabolism
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
Tandem Mass Spectrometry
Mass Spectrometry: Overview
Mass Spectrometry of Amines
Mass Spectrometry: Isotope Effect

