Catalase-linked immunosorbent pressure assay for portable quantitative analysis

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biotechnology

Background:

• C-reactive protein (CRP) is a key biomarker for cardiovascular disease risk.
• Existing detection methods may lack portability, stability, or ease of use in resource-limited settings.
• Enzyme-based immunoassays offer high sensitivity but require robust enzyme systems.

Purpose of the Study:

• To develop a portable and quantitative assay for C-reactive protein (CRP) detection.
• To establish a stable and efficient enzyme-linked immunosorbent assay using catalase.
• To demonstrate the feasibility of a pressure-based detection system for disease biomarkers.

Main Methods:

• Development of a catalase-linked immunosorbent pressure assay utilizing a gas-generation reaction.
• Modification of catalase with biotin for streptavidin-biotin based immunoassay integration.
• Optimization of biotinylation ratios and antibody concentrations for enhanced assay performance.
• Utilizing a portable pressuremeter for signal transduction and amplification in a closed chamber.

Main Results:

• The developed assay achieved a limit of detection of 1.8 nM for CRP.
• The assay demonstrated superior specificity and good accuracy compared to existing methods.
• Catalase proved to be more stable during storage and operation than PtNP nanozymes.
• The system provides a target-correlated pressure signal reading.

Conclusions:

• The catalase-linked immunosorbent pressure assay offers a stable, reproducible, and simple method for quantitative disease marker detection.
• This portable, pressure-based assay is suitable for resource-limited settings, meeting clinical needs for CRP quantification.
• Catalase's enzymatic efficiency, biocompatibility, and stability make it an advantageous enzyme for developing robust diagnostic tools.