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Updated: Jan 23, 2026
08:01
Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
Published on: September 26, 2016
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Modular imaging system: Rapid scan EPR at 800 MHz.
Oxana Tseytlin1, Priyaankadevi Guggilapu2, Andrey A Bobko1
1Biochemistry Department, West Virginia University, Morgantown, WV 26506, USA; In Vivo Multifunctional Magnetic Resonance Center at Robert C. Byrd Health Sciences Center, West Virginia University, Morgantown, WV 26506, USA.
Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|June 26, 2019
Summary
A custom-built electron paramagnetic resonance (EPR) imaging system offers high flexibility for pre-clinical studies. This versatile system enables advanced imaging techniques, including rapid scan EPR imaging, and has been validated in vivo.
Area of Science:
- Magnetic Resonance Imaging
- Pre-clinical Imaging
- Biomedical Engineering
Background:
- Electron Paramagnetic Resonance (EPR) imaging offers unique capabilities for studying biological systems.
- Existing EPR systems may lack the flexibility required for diverse experimental needs.
- Advancements in digital and analog technologies enable novel EPR system designs.
Purpose of the Study:
- To develop a highly flexible and versatile custom Electron Paramagnetic Resonance (EPR) imaging system.
- To enable both standard and novel user-defined EPR experiments.
- To demonstrate the system's capability for advanced imaging techniques like rapid scan EPR imaging.
Main Methods:
- Custom system design integrating commercial modules controlled via MATLAB.
- Utilizing frequency mixing of arbitrary waveform generator (AWG) output with constant source frequencies (SF) for flexible frequency generation (near-baseband to L-band).
- Implementing a two-stage downconversion with AWG-enabled digital auto-frequency control for resonator tuning and quadrature baseband signal generation.
- Developing a digital automatic scan control (DASC) system for stable magnetic field scans in rapid scan (RS) EPR imaging.
- Utilizing 3D printing for a custom surface loop resonator.
Main Results:
- Successful generation of a wide range of frequencies from near-baseband to L-band.
- Demonstration of multi-frequency EPR capability and automatic resonator tuning.
- Implementation and validation of rapid scan (RS) EPR imaging at 800 MHz.
- Successful in vivo imaging of a breast cancer mouse model.
- System validated using sample phantoms.
Conclusions:
- The custom-built EPR imaging system provides exceptional flexibility and versatility for pre-clinical research.
- The developed AWG-based control and DASC system enable advanced EPR techniques, including RS EPR imaging.
- The system's successful in vivo application demonstrates its potential for future pre-clinical and clinical studies.
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