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Myocardial Monophasic Action Potential Recorded by Suction Electrode for Ionic Current Studies in Zebrafish
Miguel Miranda1,2,3, José T Egaña1, Miguel L Allende2
1Institute of Biological and Medical Engineering, Pontificia Universidad Católica de Chile, Santiago, Chile.
Zebrafish
|June 28, 2019
Summary
This study introduces a novel suction electrode technique for recording myocardial monophasic action potentials (MAPs) in zebrafish. This method allows for the in situ evaluation of cardiac ion currents, crucial for understanding heart diseases.
Area of Science:
- Cardiovascular Physiology
- Electrophysiology
- Zebrafish Models
Background:
- Understanding myocardial ion currents is key to addressing cardiac pathologies like arrhythmias and ischemia.
- Conventional electrocardiography (ECG) cannot directly measure ion currents, and intracellular microelectrodes face technical challenges in beating hearts.
- Myocardial monophasic action potentials (MAPs) offer an alternative for evaluating ionic currents, mimicking intracellular recordings.
Purpose of the Study:
- To establish and validate a suction electrode technique for recording myocardial MAPs in adult zebrafish.
- To explore the feasibility of in situ evaluation of cardiac electrophysiology in zebrafish models.
- To assess the potential of this technique for studying ion channel function and cardiac disease.
Main Methods:
- Developed a suction electrode using a glass capillary tube connected to a recording system.
- Applied gentle suction to the exposed ventricle of anesthetized adult zebrafish.
- Recorded myocardial monophasic action potentials (MAPs) and conventional ECG, and induced ischemia/used lidocaine to block Na+ channels.
Main Results:
- The suction electrode successfully recorded MAPs with typical phase 0-4 morphology and low variability in amplitude and duration.
- The system could also record conventional ECG without suction.
- Ischemia and Na+ channel blockade significantly reduced MAP amplitude, confirming the technique's sensitivity to ion current changes.
Conclusions:
- The suction electrode technique is a promising and feasible method for in situ recording of myocardial ion currents in zebrafish.
- This technique provides a valuable tool for electrophysiological research in zebrafish, advancing the study of cardiac function and disease.
- The findings support the use of zebrafish as a model for investigating cardiac ion channelopathies and potential therapeutic interventions.
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