[Methods of isothermal nucleic acid amplification-based microfluidic chips for pathogen microorganism detection]

Area of Science:

• Public health microbiology
• Biotechnology
• Medical diagnostics

Background:

• Rapid detection of pathogenic microorganisms is crucial for public health disease control.
• Traditional culture-based and PCR methods have limitations, including time and equipment requirements, especially in resource-limited settings.
• Isothermal nucleic acid amplification offers an alternative but requires defined protocols for extraction, amplification, and detection.

Purpose of the Study:

• To review microfluidic detection methods for pathogens based on nucleic acid isothermal amplification.
• To summarize amplification principles, injection methods, and detection strategies.
• To highlight the advantages of integrated microfluidic systems for pathogen detection.

Main Methods:

• Integration of microfluidic technology with nucleic acid isothermal amplification.
• Optimization of chip design, sample injection modes, and detection/quantification techniques.
• Review of various microfluidic platforms for pathogen nucleic acid extraction, amplification, and detection.

Main Results:

• Microfluidic-based isothermal amplification methods enable integrated pathogen detection.
• These systems demonstrate reduced instrument dependence, lower operator skill requirements, smaller sample volumes, and increased automation.
• The reviewed methods offer enhanced capabilities for rapid screening of pathogenic microorganisms.

Conclusions:

• Microfluidic platforms combined with isothermal amplification are suitable for rapid pathogen detection in diverse environments.
• These integrated systems offer significant advantages over traditional methods, particularly for resource-limited settings.
• The technology enhances the management of infectious diseases through improved public health surveillance.