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Updated: Jan 21, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Store-Operated Calcium Entry in Mouse Cardiomyocytes
K O Gusev1, V V Vigont2, D A Grekhnev2
1Department of Ion Channels of Cell Membranes, Institute of Cytology, Russian Academy of Science, St. Petersburg, Russia. k.o.gusev@gmail.com.
Abstract:
The fluorescent dye fura-2 AM was employed to record activation of Ca2+ entry in response to a decrease in Ca2+ concentration in the endoplasmic reticulum. Using whole-cell voltage clamp technique, we revealed Ca2+ currents with an amplitude of 0.46±0.13 pA/pF that passed through selective channels with current-voltage characteristics similar to those of classical store-operated CRAC channels. These currents were sensitive to 2-APB (50 μM), an inhibitor of store-operated channels. The data suggest that store-operated calcium entry is a characteristic feature of mature ventricular cardiomyocytes. Pathological alterations in store-operated Ca2+ entry can be implicated in the development of heart diseases.
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