Mass Spectrometry as a Complementary Approach for Noncovalently Bound Complexes Based on Cyclodextrins.

Area of Science:

• Pharmaceutical Science
• Analytical Chemistry
• Supramolecular Chemistry

Background:

• Drug development faces challenges with solubility, stability, and bioavailability.
• Molecular encapsulation in cyclodextrins (CDs) offers a solution by forming inclusion complexes (ICs).
• These non-covalent ICs are vital for the pharmaceutical industry.

Purpose of the Study:

• To review methods for preparing and characterizing cyclodextrin inclusion complexes (CDs ICs).
• To focus on mass spectrometry (MS) applications for detecting non-covalent interactions and binding selectivity.
• To present detailed MS investigations of specific CD ICs.

Main Methods:

• Review of cyclodextrin inclusion complex preparation techniques.
• Emphasis on mass spectrometry (MS) for non-covalent interaction analysis.
• Detailed case studies of MS analysis for antifungal, antioxidant, and dye inclusion complexes.

Main Results:

• Mass spectrometry effectively detects non-covalent interactions in cyclodextrin inclusion complexes.
• MS provides insights into guest molecule binding selectivity with cyclodextrins.
• Specific examples demonstrate successful MS analysis of challenging inclusion complexes.

Conclusions:

• Cyclodextrin inclusion complexes are a promising strategy for improving drug properties.
• Mass spectrometry is an indispensable tool for characterizing these complexes and their interactions.
• MS analysis, despite challenges, offers valuable data for pharmaceutical applications.