Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

4.6K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
4.6K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.8K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.8K
Vibrating Concrete01:19

Vibrating Concrete

379
Mechanical vibrators are instrumental in compacting newly poured concrete within formwork and around reinforcements. This process is essential to eliminate trapped air pockets and establish a dense concrete mass. One widely used method is vibrating by internal vibrators, often referred to as a poker vibrator or immersion vibrator. It is rapidly inserted through the full depth of the freshly laid concrete and slightly extends into the layer below it (which remains in a plastic state). Consistent...
379
Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

14.7K
Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
14.7K
Molecular Orbital Theory I02:35

Molecular Orbital Theory I

47.1K
Overview of Molecular Orbital Theory
47.1K
Phase Diagrams02:39

Phase Diagrams

49.0K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
49.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photonic decision making using optical frequency difference detection in mutually-coupled semiconductor lasers.

Optics express·2026
Same author

Compressive multi-beam scanning transmission electron microscopy.

Ultramicroscopy·2026
Same author

Compressive event camera.

Optics express·2025
Same author

Bidirectional quantitative scattering microscopy.

Nature communications·2025
Same author

Remote training of a reservoir computer via digital twins.

Chaos (Woodbury, N.Y.)·2025
Same author

Ludwig-Soret microscopy with the vibrational photothermal effect.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026
Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026
Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026
Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026
Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026
Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
08:58

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

Published on: November 19, 2018

13.0K

Quantitative phase imaging with molecular vibrational sensitivity.

Miu Tamamitsu, Keiichiro Toda, Ryoichi Horisaki

    Optics Letters
    |August 2, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Quantitative phase imaging (QPI) now offers chemical sensitivity by integrating molecular vibrational (MV) microscopy. This new technique enables high-speed, label-free imaging of both morphology and molecular information simultaneously.

    More Related Videos

    A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
    12:48

    A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

    Published on: February 16, 2018

    7.8K
    A Strategy for Sensitive, Large Scale Quantitative Metabolomics
    14:18

    A Strategy for Sensitive, Large Scale Quantitative Metabolomics

    Published on: May 27, 2014

    21.7K

    Related Experiment Videos

    Last Updated: Jan 21, 2026

    Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning
    08:58

    Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

    Published on: November 19, 2018

    13.0K
    A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
    12:48

    A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells

    Published on: February 16, 2018

    7.8K
    A Strategy for Sensitive, Large Scale Quantitative Metabolomics
    14:18

    A Strategy for Sensitive, Large Scale Quantitative Metabolomics

    Published on: May 27, 2014

    21.7K

    Area of Science:

    • Biophotonics
    • Spectroscopy
    • Microscopy

    Background:

    • Quantitative phase imaging (QPI) provides label-free, morphology-based analysis of transparent samples.
    • QPI lacks chemical sensitivity, limiting its diagnostic capabilities.
    • Molecular vibrational (MV) microscopy offers chemical contrast but is often slow or requires labels.

    Purpose of the Study:

    • To develop a wide-field molecular vibrational microscopy technique integrated within the QPI framework.
    • To achieve simultaneous, label-free acquisition of both quantitative morphology and molecular information.
    • To enhance diagnostic capabilities for optically transparent specimens.

    Main Methods:

    • Integration of mid-infrared (MIR) photothermal effect into QPI.
    • Utilizing a high-energy MIR pulse source for excitation.
    • Achieving wide-field imaging with high spatial resolution and low laser fluence.

    Main Results:

    • Demonstrated MIR spectroscopic performance comparable to conventional spectrometers in the 1450–1640 cm-1 range.
    • Achieved wide-field molecular imaging of a silica-polystyrene bead mixture (100 μm × 100 μm) at 1 frame/sec.
    • Obtained a spatial resolution of 430 nm with significantly reduced photodamage (∼10 pJ/μm2 fluence).

    Conclusions:

    • The developed technique enables high-speed, label-free, simultaneous acquisition of morphology and molecular vibrational contrast.
    • This advancement offers new insights into optically transparent complex dynamics.
    • Potential for improved, morphology-based diagnosis in biological and material sciences.