Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.8K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.8K
Chromatin Packaging02:21

Chromatin Packaging

21.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
21.8K
The Nucleosome Core Particle02:10

The Nucleosome Core Particle

14.1K
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
14.1K
Angular Momentum: Single Particle01:10

Angular Momentum: Single Particle

7.6K
Angular momentum is directed perpendicular to the plane of the rotation, and its magnitude depends on the choice of the origin. The perpendicular vector joining the linear momentum vector of an object to the origin is called the “lever arm.” If the lever arm and linear momentum are collinear, then the magnitude of the angular momentum is zero. Therefore, in this case, the object rotates about the origin such that it lies on the rim of the circumference defined by the lever arm...
7.6K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.7K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.7K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.3K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical outcomes of trifluridine/tipiracil plus bevacizumab versus trifluridine/tipiracil or regorafenib in metastatic colorectal cancer: a multicenter cohort study.

ESMO open·2026
Same author

Exploring the differences in the tumor microenvironment and immuno-oncologic targets in pancreatic ductal adenocarcinomas (PDAC) according to <i>KRAS</i> mutational status.

ESMO gastrointestinal oncology·2026
Same author

Unveiling the functional connectivity of astrocytic networks with AstroNet, a graph reconstruction algorithm coupled to image processing.

Communications biology·2025
Same author

Mapping general anesthesia states based on electro-encephalogram transition phases.

NeuroImage·2023
Same author

Publisher Correction: Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD.

Nature structural & molecular biology·2023
Same author

Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD.

Nature structural & molecular biology·2023
Same journal

Genetic suppressors as new therapeutic targets for Mendelian diseases.

Trends in genetics : TIG·2026
Same journal

Beyond housekeeping: snRNA diversity, regulation, and human disease.

Trends in genetics : TIG·2026
Same journal

Rethinking mitochondrial metabolism: Intraindividual variability meets population constraints.

Trends in genetics : TIG·2026
Same journal

A role for epigenetics in rapid adaptation.

Trends in genetics : TIG·2026
Same journal

The myth of asexual fungi.

Trends in genetics : TIG·2026
Same journal

Rethinking molecular evolution through protein language model embeddings.

Trends in genetics : TIG·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

Automated Rat Single-Pellet Reaching with 3-Dimensional Reconstruction of Paw and Digit Trajectories
07:52

Automated Rat Single-Pellet Reaching with 3-Dimensional Reconstruction of Paw and Digit Trajectories

Published on: July 10, 2019

15.0K

Advances Using Single-Particle Trajectories to Reconstruct Chromatin Organization and Dynamics.

O Shukron1, A Seeber2, A Amitai3

  • 1Group of Data Modeling, Computational Biology and Predictive Medicine, Institut de Biologie, CNRS/INSERM/PSL Ecole Normale Supérieure, Paris, 75005, France.

Trends in Genetics : TIG
|August 3, 2019
PubMed
Summary
This summary is machine-generated.

Researchers review statistical methods to analyze single-particle trajectories and reconstruct chromatin organization and dynamics. This helps understand chromatin reorganization in response to cellular stress like DNA damage.

Keywords:
Locianalysischromatinpolymer modelssingle-particle trajectoriesstatistical methods

More Related Videos

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

23.9K
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

10.0K

Related Experiment Videos

Last Updated: Jan 21, 2026

Automated Rat Single-Pellet Reaching with 3-Dimensional Reconstruction of Paw and Digit Trajectories
07:52

Automated Rat Single-Pellet Reaching with 3-Dimensional Reconstruction of Paw and Digit Trajectories

Published on: July 10, 2019

15.0K
Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
12:10

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Published on: March 28, 2011

23.9K
Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
10:38

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles

Published on: September 21, 2018

10.0K

Area of Science:

  • Molecular Biology
  • Biophysics
  • Genomics

Background:

  • Chromatin organization is complex and not fully understood.
  • Cellular stress, such as DNA damage, can induce chromatin reorganization.
  • Understanding chromatin dynamics is crucial for cellular function.

Purpose of the Study:

  • To review statistical methods for extracting biophysical parameters from single-particle trajectories.
  • To explain how to quantify and describe chromatin motion and dynamics.
  • To discuss polymer models for reconstructing chromatin structure.

Main Methods:

  • Analysis of in vivo single-locus and multi-locus trajectories.
  • Extraction and interpretation of biophysical parameters describing chromatin motion.
  • Application of polymer models and simulation software for chromatin structure reconstruction.

Main Results:

  • Biophysical parameters can quantify chromatin dynamics and function.
  • Timescales of recurrent locus encounters can be extracted and interpreted.
  • Sampling rate affects parameter estimation.

Conclusions:

  • Chromatin organization and dynamics can be reconstructed from locus trajectories.
  • Polymer models can predict chromatin structure and behavior.
  • Statistical methods provide insights into chromatin reorganization under cellular stress.