Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

20.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K
DNA Replication02:40

DNA Replication

58.8K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
58.8K
DNA-only Transposons02:57

DNA-only Transposons

17.3K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.3K
Directing Effect of Substituents: meta-Directing Groups01:09

Directing Effect of Substituents: meta-Directing Groups

5.8K
Substituents on the benzene ring that direct an incoming electrophile to undergo substitution at the meta position are called meta directors. All meta directors either have a positive charge on the atom directly bonded to the ring or a partial positive charge. These groups function by withdrawing electrons from the ring through inductive and resonance effects. Consider the carbocation intermediates formed upon the addition of an electrophile on nitrobenzene at the...
5.8K
DNA Topoisomerases02:02

DNA Topoisomerases

35.1K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
35.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Printing Microarrays.

Cold Spring Harbor protocolsยท2019
Same author

Blocking Polylysines on Homemade Microarrays.

Cold Spring Harbor protocolsยท2019
Same author

Hybridization to Homemade Microarrays.

Cold Spring Harbor protocolsยท2019
Same author

Round A/Round B Amplification of DNA for Microarrays.

Cold Spring Harbor protocolsยท2019
Same author

T7 Linear Amplification of DNA (TLAD) for Microarrays.

Cold Spring Harbor protocolsยท2019
Same author

Indirect Labeling of DNA for Microarrays.

Cold Spring Harbor protocolsยท2019

Related Experiment Video

Updated: Jan 21, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

10.4K

Direct Cyanine-dCTP Labeling of DNA for Microarrays.

Oliver Rando

    Cold Spring Harbor Protocols
    |August 3, 2019
    PubMed
    Summary

    Direct DNA labeling using Cy-dCTP offers a simple and rapid method for DNA labeling. This Klenow labeling protocol is effective for various applications like copy-number variation detection and chromatin immunoprecipitation-chip assays.

    Area of Science:

    • Molecular Biology
    • Genomics
    • Biochemistry

    Background:

    • Direct DNA labeling is crucial for various genomic analyses.
    • Existing methods can be complex or time-consuming.

    Purpose of the Study:

    • To present a simple and fast method for direct DNA labeling using Cy-dCTP.
    • To highlight the utility of this method in diverse molecular biology applications.

    Main Methods:

    • Incorporation of Cy-dCTP into DNA using a standard Klenow labeling protocol.
    • Separation of labeled nucleotides from unreacted Cy-dCTP.
    • Hybridization of Cy3- and Cy5-labeled DNA materials.

    Main Results:

    • Successful direct DNA labeling with Cy-dCTP.

    More Related Videos

    Identification of Mycobacterium Species by DNA Microarray Chip Method
    06:27

    Identification of Mycobacterium Species by DNA Microarray Chip Method

    Published on: June 24, 2025

    541
    Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
    06:58

    Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

    Published on: February 2, 2018

    9.2K

    Related Experiment Videos

    Last Updated: Jan 21, 2026

    ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
    09:32

    ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

    Published on: June 9, 2011

    10.4K
    Identification of Mycobacterium Species by DNA Microarray Chip Method
    06:27

    Identification of Mycobacterium Species by DNA Microarray Chip Method

    Published on: June 24, 2025

    541
    Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
    06:58

    Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

    Published on: February 2, 2018

    9.2K
  • Demonstrated suitability for copy-number variation detection.
  • Validated application in nucleosome mapping and chromatin immunoprecipitation-chip assays.

    • Conclusions:

    • Direct DNA labeling with Cy-dCTP is an efficient and straightforward technique.
    • This method provides a valuable tool for location analysis and genomic studies.