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Related Experiment Video

Updated: Jan 20, 2026

Performing Custom MicroRNA Microarray Experiments
07:04

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Printing Microarrays.

Oliver Rando

    Cold Spring Harbor Protocols
    |September 5, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Laboratories can save costs by printing their own microarrays, which is a straightforward process. This protocol details a typical workflow for microarray printing using a spotting robot and contact steel quill pins.

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    Area of Science:

    • Biotechnology
    • Molecular Biology
    • Genomics

    Background:

    • Commercial microarray production is common, but in-house printing offers potential cost benefits for labs with high demand.
    • Microarray printing is a technically feasible process for smaller laboratories.

    Purpose of the Study:

    • To provide a protocol for in-house microarray printing.
    • To demonstrate the cost-effectiveness of self-produced microarrays for laboratories with extensive needs.

    Main Methods:

    • Utilizes a spotting robot equipped with contact steel quill pins.
    • Involves distributing oligonucleotides into 384-well plates.
    • Prints oligonucleotides onto polylysine-coated microarray slides.

    Main Results:

    • A typical workflow for microarray printing is detailed.
    • The protocol is adaptable based on specific robot manufacturer instructions.

    Conclusions:

    • In-house microarray printing is a viable and potentially cost-effective option for laboratories.
    • This protocol serves as a guide for implementing self-sufficient microarray production.