Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

20.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
20.8K
Labeling DNA Probes03:31

Labeling DNA Probes

9.3K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.3K
Indirect Motor Pathways01:22

Indirect Motor Pathways

3.1K
The indirect motor or extrapyramidal pathways originate in the brainstem, the lower portion of the brain that connects it to the spinal cord. They consist of several distinct tracts, each with specialized functions. The four main tracts of the indirect motor pathways are the vestibulospinal tract, the reticulospinal tract, the tectospinal tract, and the rubrospinal tract.
The vestibulospinal tract originates in the vestibular nuclei of the brainstem. The vestibular system detects changes in...
3.1K
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

1.8K
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
1.8K
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

1.4K
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
1.4K
Indirect-Acting Cholinergic Agonists: Pharmacokinetics01:22

Indirect-Acting Cholinergic Agonists: Pharmacokinetics

1.6K
Indirect-acting cholinergic agonists, or anticholinesterases, enhance the body's cholinergic activity by inhibiting acetylcholine's breakdown. They are categorized as reversible or irreversible agents based on their mechanism of action. They are further classified into short-acting, intermediate-acting, and long-acting agents based on their duration of action.
Reversible agents containing quaternary amines, such as neostigmine and edrophonium, are not easily absorbed orally because they...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Printing Microarrays.

Cold Spring Harbor protocols·2019
Same author

Blocking Polylysines on Homemade Microarrays.

Cold Spring Harbor protocols·2019
Same author

Hybridization to Homemade Microarrays.

Cold Spring Harbor protocols·2019
Same author

Round A/Round B Amplification of DNA for Microarrays.

Cold Spring Harbor protocols·2019
Same author

T7 Linear Amplification of DNA (TLAD) for Microarrays.

Cold Spring Harbor protocols·2019
Same author

Direct Cyanine-dCTP Labeling of DNA for Microarrays.

Cold Spring Harbor protocols·2019

Related Experiment Video

Updated: Jan 21, 2026

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
09:32

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

Published on: June 9, 2011

10.4K

Indirect Labeling of DNA for Microarrays.

Oliver Rando

    Cold Spring Harbor Protocols
    |August 3, 2019
    PubMed
    Summary
    This summary is machine-generated.

    Indirect DNA labeling is cheaper than direct labeling but takes about two hours longer. This cost-effective method is suitable for researchers prioritizing budget over time.

    More Related Videos

    Identification of Mycobacterium Species by DNA Microarray Chip Method
    06:27

    Identification of Mycobacterium Species by DNA Microarray Chip Method

    Published on: June 24, 2025

    541
    DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
    09:27

    DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

    Published on: March 15, 2011

    38.6K

    Related Experiment Videos

    Last Updated: Jan 21, 2026

    ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
    09:32

    ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

    Published on: June 9, 2011

    10.4K
    Identification of Mycobacterium Species by DNA Microarray Chip Method
    06:27

    Identification of Mycobacterium Species by DNA Microarray Chip Method

    Published on: June 24, 2025

    541
    DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
    09:27

    DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

    Published on: March 15, 2011

    38.6K

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • DNA labeling is crucial for various molecular biology applications, including genetic analysis and diagnostics.
    • Direct and indirect labeling protocols offer different approaches to attaching labels to DNA molecules.

    Purpose of the Study:

    • To describe and evaluate an indirect DNA labeling protocol.
    • To compare the indirect DNA labeling protocol with direct labeling in terms of cost and time.

    Main Methods:

    • The study details an indirect DNA labeling protocol.
    • Key steps and reagents for indirect DNA labeling are outlined.

    Main Results:

    • The indirect DNA labeling protocol takes approximately 2 hours longer than direct labeling.
    • Indirect DNA labeling is significantly more cost-effective, saving hundreds of dollars compared to direct labeling.

    Conclusions:

    • Indirect DNA labeling presents a viable, budget-friendly alternative to direct labeling.
    • Researchers can choose between direct and indirect methods based on their specific constraints of time and cost.