Related Experiment Video
Updated: Jan 21, 2026

A Tissue Clearing Method for Neuronal Imaging from Mesoscopic to Microscopic Scales
Published on: May 10, 2022
Simple method to generate calibrated synthetic smoke-like atmospheres at microscopic scale
Jose M Nadal-Serrano1, Elia Gomez G de la Pedrosa2, Marisa Lopez-Vallejo1
1ETSI Telecomunicación, Universidad Politécnica de Madrid, Madrid, Spain.
This study introduces a novel method for creating customizable microscopic smoke-like atmospheres using discrete microspheres. This technique enables reproducible synthetic media for calibrating optical instruments and sensors.
Area of Science:
- Materials Science
- Optical Engineering
- Aerosol Science
Background:
- Traditional artificial smoke focuses on macroscopic properties, limiting microscopic applications.
- Need for reproducible, customizable synthetic media for precise optical measurements.
Purpose of the Study:
- To present a simple method for generating microscopic, customizable smoke-like atmospheres.
- To demonstrate the creation of synthetic, reproducible media using monodisperse microspheres.
Main Methods:
- Utilizing monodisperse microspheres with known geometrical and optical properties.
- Analyzing aerosol nebulization, particle carrier selection (methanol vs. ethanol), and chamber design.
- Parameterizing synthetic media for controlled smoke-like atmospheres.
Main Results:
- Proof-of-concept for generating customizable microscopic smoke-like atmospheres.
- Analysis of practical considerations for reproducible aerosol generation.
- Comparison of methanol and ethanol as carriers for polystyrene microsphere nebulization.
Conclusions:
- The developed method offers a new approach for creating standard reference media.
- Potential applications include calibration of smoke detectors, exhaust sensors, and optical instruments for dust or lighting.
Related Concept Videos
Glassware Calibration
Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...
Instrument Calibration
Analytical Balance Calibration
An analytical balance measures mass and requires regular calibration to...
Definition and Measurement of Pressure: Atmospheric Pressure, Barometer, and Manometer
pH Scale
Synthetic Biology
Golden rice
Golden rice is a genetically modified...
Plotting and Calibrating the Root Locus
The maximum gain occurs at the breakaway points between open-loop poles on the real axis, while the minimum gain is...

