Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Steps in the Modeling Process01:14

Steps in the Modeling Process

647
Albert Bandura's theory of observational learning identifies four critical processes: attention, retention, motor reproduction, and reinforcement or motivation.
Attention is the first necessary component for observational learning. It involves focusing on what the model is doing and saying. For example, if you decide to take a drawing class to enhance your skills, you need to pay close attention to the instructor's words and hand movements. The characteristics of the model significantly...
647
Rate-Determining Steps03:08

Rate-Determining Steps

36.8K
Relating Reaction Mechanisms
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
36.8K
Steps in Outbreak Investigation01:18

Steps in Outbreak Investigation

494
In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
494
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.7K
Multi-Step Reactions02:31

Multi-Step Reactions

8.6K
Chemical reactions often occur in a stepwise fashion involving two or more distinct reactions taking place in a sequence. A balanced equation indicates the reacting species and the product species, but it reveals no details about how the reaction occurs at the molecular level. The reaction mechanism (or reaction path) provides details regarding the precise, step-by-step process by which a reaction occurs. Each of the steps in a reaction mechanism is called an elementary reaction. These...
8.6K
Steps for Free-Body Diagram01:22

Steps for Free-Body Diagram

3.2K
When it comes to studying the behavior of objects in mechanics, one of the most important tools available is the free-body diagram. Consider a simple example of a system of two blocks coupled by a massless string over a frictionless pulley. Block 1 is sliding over a table pulled by block 2 as block 2 falls under gravity.
To find the acceleration of the system, it is first necessary to calculate the net force on the system. In order to accomplish this, a free-body diagram can be created to...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell Proliferation High-Content Screening on Adherent Cell Cultures.

Methods in molecular biology (Clifton, N.J.)·2019
Same author

A method for resolving occlusions when multitracking individuals in a shoal.

Behavior research methods·2014
Same author

Assessment of the implantation of day-2 human embryos by morphometric nonsubjective parameters.

Fertility and sterility·2014
Same author

Delayed fatherhood in mice decreases reproductive fitness and longevity of offspring.

Biology of reproduction·2008
Same author

Long-term effects of delayed fatherhood in mice on postnatal development and behavioral traits of offspring.

Biology of reproduction·2008
Same author

Beneficial effects of dithiothreitol on relative levels of glutathione S-transferase activity and thiols in oocytes, and cell number, DNA fragmentation and allocation at the blastocyst stage in the mouse.

Molecular reproduction and development·2006
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
14:28

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Published on: July 15, 2020

8.3K

Main Steps in Image Processing and Quantification: The Analysis Workflow.

José F Pertusa1, Jose M Morante-Redolat2,3,4

  • 1Departamento de Biología Celular, Biología Funcional y Antropología Física, Universitat de València, Burjassot, Spain. pertusa@uv.es.

Methods in Molecular Biology (Clifton, N.J.)
|August 22, 2019
PubMed
Summary
This summary is machine-generated.

Effective image analysis requires careful planning and a structured workflow. This guide outlines key strategies for researchers to overcome image manipulation challenges and obtain unbiased quantitative data.

Keywords:
Image analysisImage processingImage quantificationMain step’s analysis

More Related Videos

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

679
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
05:35

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Published on: September 20, 2022

4.2K

Related Experiment Videos

Last Updated: Jan 20, 2026

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
14:28

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images

Published on: July 15, 2020

8.3K
High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples
11:24

High-throughput Imaging and Analysis Workflow for Evaluating Skin Cell Phenotypes and Proliferation States in Tissue Samples

Published on: October 31, 2025

679
An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome
05:35

An Integrated Workflow of Identification and Quantification on FDR Control-Based Untargeted Metabolome

Published on: September 20, 2022

4.2K

Area of Science:

  • Image analysis
  • Quantitative data acquisition
  • Scientific research methodologies

Background:

  • Extensive growth in image processing tools and algorithms.
  • Need for strategic planning in image analysis.
  • Challenges in image manipulation impacting data integrity.

Purpose of the Study:

  • To provide strategies for successful image analysis.
  • To outline the essential milestones in an image processing workflow.
  • To guide researchers in obtaining unbiased quantitative data.

Main Methods:

  • Review of existing image processing strategies.
  • Reflection on critical steps in the image analysis workflow.
  • Development of a structured approach to image data processing.

Main Results:

  • Identification of key challenges in image manipulation.
  • A proposed sequence of operations for effective image analysis.
  • Emphasis on the importance of planning for unbiased results.

Conclusions:

  • Strategic planning is crucial for effective image analysis.
  • A well-defined workflow overcomes image manipulation issues.
  • The presented strategies facilitate unbiased quantitative data acquisition.