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Updated: Jan 20, 2026

Isolation and Identification of Vascular Endothelial Cells from Distinct Adipose Depots for Downstream Applications
Published on: June 10, 2022
Protective effect of green tea on tunica adventitia and endothelial changes resulting from depot medroxy progesterone
Nora Veri1, Emilda As1, Cut Mutiah1
1Midwifery Study Program, Ministry of Health Polytechnic of Langsa, Aceh, Special Region of Aceh, Indonesia.
Objective:
This study aimed to analyse the effects of green tea in inhibiting uterine atrophy and vascular changes due to the use of depot medroxy progesterone acetate (DMPA).
Methods:
Twenty-five female Wistar rats aged one to two months were randomly assigned to five treatment groups: control group, DMPA-induced group, and DMPA-induced group orally treated with green tea extract (at 10.8 mg/day, 21.6 mg/day, or 43.2 mg/day). Histologic analysis of uterine and vascular tissues was performed with haematoxylin-eosin staining.
Results:
DMPA decreased the thickness of endometrium and tunica adventitia, as well as significantly decreased endothelial cell count (p < 0.05). DMPA-induced decreases in the thickness of tunica adventitia and endothelial cell count could be significantly inhibited by green tea extract (p < 0.05).
Conclusion:
This study concluded that DMPA triggered the depletion of uterine endometrium and vascular tunica adventitia and decreased endothelial cell count. Green tea extract at the highest dose normalized tunica adventitia and endothelial changes to the basal value.
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